CAS Number: 2050-87-5
Molecular formula: C6H10S3
Molecular weight: 178.34
EINECS number: 218-107-8
Customs code 29309090
Boiling point: 229.5℃ at 760mmHg
Storage conditions: 2～8℃
Flash point: 87.8℃
Diallyl Trisulfide Introduction
Diallyl trisulfide is isolated from garlic. It can inhibit the growth of Penicillium dilatatum (MFC99 value: ≤ 90 μg/mL), and promote cell apoptosis by generating reactive oxygen species (ROS) and destroying cell ultrastructure. It has anti-cancer ability.
Chemical nature yellow liquid. Has a disgusting smell. The boiling point is 112～120℃(2133Pa), or 95～97℃(667Pa) or 70℃(133Pa). Insoluble in water and ethanol, but miscible in ether. Natural products exist in onions, garlic, etc.
Application standard: The fragrance ingredients used in the preparation of flavors shall not exceed the maximum allowable usage and maximum allowable residues in GB 2760
Diallyl trisulfide is a yellow liquid with an offensive odor. It has a boiling point of 112-120°C (2133Pa) or 95-97°C (667Pa). It is insoluble in water and ethanol, but miscible in ether. The main uses are pharmaceutical intermediates, food additives, and feed additives.
Diallyl Trisulfide Function
Studies have found that diallyl trisulfide (the main active substance of garlic essential oil) interferes with the mating behavior of wheat moths, thereby reducing the egg production of wheat moths. At the same time, diallyl trisulfide (the main active substance of garlic oil) Thioether regulated the expression of many reproduction-related genes in wheat moth under sub-lethal dose conditions.
Studies have found that diallyl trisulfide has a good bactericidal effect on Helicobacter pylori in a floating state. The research on H. pylori biofilm has mostly focused on its structural characteristics, and there has been no report on the research of drug resistance. In this study, diallyl trisulfide was used, and clarithromycin, one of the commonly used antibiotics in the treatment of H. pylori infection was selected, and a biofilm model of H. pylori was constructed in vitro to explore diallyl trisulfide. And clarithromycin on the bactericidal effect of H. pylori biofilm, and compare the bactericidal effects of the two.
The results of this study show that H. pylori has a strong sensitivity to clarithromycin and diallyl trisulfide in a floating state, but after H. pylori forms a biofilm, it is resistant to clarithromycin Significantly enhanced. Although compared with the control group, the activity decrease of the clarithromycin treatment group was statistically different (P＜0.01), but when clarithromycin was used with a concentration of one thousand times the minimum inhibitory concentration, the bactericidal effect did not increase effectively, suggesting clarithromycin The element cannot reach the deep layer of the biofilm structure well, and can only kill the shallow layer of bacteria.
Compared with clarithromycin, the use of a lower concentration (50μg/mL) of diallyl trisulfide has a good killing effect on H. pylori in the biofilm state, and as the drug concentration increases, The bactericidal effect is gradually enhanced. The CLSM results in this study showed that after the diallyl trisulfide treatment, larger pores appeared in the biofilm structure and the structure became loose. This is probably because the organic sulfur compounds can freely pass through the phospholipid bilayer of the bacterial cell wall, which is easier to destroy the extracellular polymer in the biofilm structure, so that it can better penetrate into the deep layer of the biofilm and play a bactericidal effect.
Studies have found that diallyl trisulfide can inhibit platelet aggregation. The specific mechanism is: diallyl trisulfide concentration-dependently inhibits platelet aggregation induced by thrombin and collagen-related polymorphisms, with IC50 of 10 ug / ml and 4.3 ug/ml. Diallyl trisulfide obviously inhibits the formation of thromboxane when the cells are intact, but does not inhibit the formation of thromboxane under cell-free conditions, indicating that its inhibition of thromboxane formation is not a direct inhibition Also oxidase and thromboxane forming enzyme, but an indirect effect.
Diallyl trisulfide strongly inhibits the influx and outflow of calcium ions induced by thrombin and collagen-related polymorphisms, but has no inhibitory effect on the influx of calcium ions induced by the calcium store ATPase inhibitor Thapsigargin, indicating that diallyl trisulfide Thioether only inhibits the mobilization of calcium ions mediated by IP3. Diallyl trisulfide cannot inhibit the production of IP3, but it can prevent the binding of IP3 to its receptor; it has no obvious effect on the increase of intracellular cAMP and cGMP.
Studies have found that DATS combined with DDP has a positive effect on improving nephrotoxicity. By constructing a DDP-induced BALb/c mouse kidney injury model to study the prevention and treatment effects of DATS, the results show that after DATS pretreatment and administration, it can effectively reduce the abnormal renal function caused by DDP, such as regulating the expression of inflammatory factors and repairing kidney tissue Injury and inflammation around the mesorenic tubules.